| September 1990 | First gene therapy recipient | first | Ashanti DeSilva |
| 24 July 1990 | Fastest walk 10,000 metres (female) | 41/56.23 minute(s):second(s) | Nadezhda Ryashkina |
| 22 July 1990 | Fastest 400 m joggling with three objects - women | 1/16 minute(s):second(s) | Christa Rypins |
| 22 July 1990 | Fastest 100 m joggling with three objects - women | | Sandy Brown |
| 22 July 1990 | Fastest mile juggling three objects whilst running in relay (female) | 6/54.1 minute(s):second(s) | Sandy Brown, Kay Caskey, Kathy Glynn, Laurie Young |
| 27 May 1990 | Fastest time to complete the Indianapolis 500 race | 2/41/18.404 hour(s):minute(s):second(s) | Arie Luyendyk |
| 20 May 1990 | Farthest shot put (male) | 23.12 metre(s) | Randy Barnes |
| 24 April 1990 | Largest space telescope | 11 tonne(s)/metric ton(s) | Unknown |
| 29 March 1990 | Oldest Oscar winner as Best Actress | | Jessica Tandy |
| 28 February 1990 | Most penalties in an NHL game | | Edmonton Oilers, LA Kings |
| 01 February 1990 | Most expensive magic show | 28000000 US dollar(s) | Siegfried and Roy at the Mirage |
| 20 January 1990 | Oldest Golden Globe winner - actress | | Jessica Tandy |
| 1990 | First successful craniopagus separation | | Vilija & Vatalija Tamulevicius |
| 1990-1993, 1997, 1998 | Most wins of Entertainer of the Year at the Academy of Country Music Awards | 6 total number | Garth Brooks |
| 1984-1987, 1990, 1991, 1997 | Most wins of Female Vocalist of the Year at the Academy of Country Music Awards | | Reba McEntire |
| 1990 | First fully digital feature film | first | The Rescuers Down Under |
| 1990 | Most Oscar nominations for best director for an actor in the same film | 5 total number | Woody Allen |
| 1990 | First Grammy-winning artist to be stripped of their award | | Milli Vanilli |